High-precision electrical AFM modes for biological applications
Electrical modes of Atomic Force Microscopy (AFM) allow the high-resolution mapping of surface charges on a sample with nanometer precision. A particular measurement challenge is to perform such modes on biological samples (tissue extracts, biomolecules, biomembranes, etc). To this end, the project aims to investigate different preparation and measurement approaches and a specific goal is to determine alterations of surface charge of biological fibers caused by the reaction with sugar, which has important implications in medicine and cell biology.
Figure 1: KFM surface potential map of a collagen fibril on graphite. The bright spots show areas of positive charge.
- Determine the influence of humidity on ionised, chemical surface groups
- Investigate the charge of glycated protein fibrils
- Kohl, D., P. Mesquida and G. Schitter (2017). “Quantitative AC – Kelvin Probe Force Microscopy.” Microelectronic Engineering 176: 28-32.
- P.Mesquida, D.Kohl, O.G.Andriotis, P.J.Thurner, M.J.Duer, S.B.Bansode, G.Schitter, Kelvin-probe Force Microscopy to map glycation of proteins, AFM Biomed Conference, Krakow 2017.